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Suppression of the ecdysteroid‐triggered growth arrest by a novel Drosophila membrane steroid binding protein
Author(s) -
Fujii-Taira Ikuko,
Yamaguchi Shinji,
Iijima Ryoko,
Natori Shunji,
Homma Koichi J.
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.12.056
Subject(s) - ecdysteroid , microbiology and biotechnology , ecdysone , biology , extracellular , steroid hormone , ecdysone receptor , receptor , steroid , medicine , endocrinology , hormone , biochemistry , transcription factor , nuclear receptor , gene
Ecdysteroid is a crucial steroid hormone in insects, especially during metamorphosis. Here, we show that the Drosophila membrane steroid binding protein (Dm_MSBP) is a novel structural homolog of the vertebrate membrane‐bound receptor component for progesterone. Dm_MSBP exhibited binding affinity to ecdysone when expressed on the cell surface of Drosophila S2 cells. In S2 cells, the stable overexpression of Dm_MSBP suppressed the growth arrest triggered by 20‐hydroxyecdysone and prevented the temporal activation of extracellular signal‐regulated kinase proteins. These results suggest that Dm_MSBP is a membranous suppressor to ecdysteroid and blocks the signaling by binding it in extracellular fluid.