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The NtAMI1 gene functions in cell division of tobacco BY‐2 cells in the presence of indole‐3‐acetamide
Author(s) -
Nemoto Keiichirou,
Hara Masamitsu,
Suzuki Masashi,
Seki Hikaru,
Muranaka Toshiya,
Mano Yoshihiro
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.12.049
Subject(s) - nicotiana tabacum , auxin , agrobacterium , arabidopsis thaliana , gene , indole test , acetamide , rna interference , biology , cell division , indole 3 acetic acid , microbiology and biotechnology , chemistry , cell , rna , biochemistry , transgene , astrobiology , mutant
Tobacco ( Nicotiana tabacum ) Bright Yellow‐2 (BY‐2) cells can be grown in medium containing indole‐3‐acetamide (IAM). Based on this finding, the NtAMI1 gene, whose product is functionally equivalent to the AtAMI1 gene of Arabidopsis thaliana and the aux2 gene of Agrobacterium rhizogenes , was isolated from BY‐2 cells. Overexpression of the NtAMI1 gene allowed BY‐2 cells to proliferate at lower concentrations of IAM, whereas suppression of the NtAMI1 gene by RNA interference (RNAi) caused severe growth inhibition in the medium containing IAM. These results suggest that IAM is incorporated into plant cells and converted to the auxin, indole‐3‐acetic acid, by NtAMI1 .