Premium
Cu,Zn‐superoxide dismutase is necessary for proper function of VDAC in Saccharomyces cerevisiae cells
Author(s) -
Karachitos Andonis,
Galganska Hanna,
Wojtkowska Malgorzata,
Budzinska Malgorzata,
Stobienia Olgierd,
Bartosz Grzegorz,
Kmita Hanna
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.12.045
Subject(s) - voltage dependent anion channel , saccharomyces cerevisiae , sod1 , superoxide dismutase , chemistry , mitochondrion , microbiology and biotechnology , yeast , biochemistry , vdac1 , oxidative stress , biology , gene , bacterial outer membrane , escherichia coli
Available data suggest that a copper‐and zinc‐containing dismutase (CuZnSOD) plays a significant role in protecting eukaryotic cells against oxidative modifications which may contribute to cell aging. Here we demonstrated that depletion of CuZnSOD in Saccharomyces cerevisiae cells (Δ sod1 cells) affected distinctly channel activity of VDAC (voltage dependent anion selective channel) and resulted in a moderate reduction in VDAC levels as well as in levels of protein crucial for VDAC import into mitochondria, namely Tob55/Sam50 and Tom40. The observed alterations may result in mitochondriopathy and subsequently in the shortening of the replicative life span observed for S. cerevisiae Δ sod1 cells.