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Down‐regulation of adenine nucleotide translocase 3 and its role in camptothecin‐induced apoptosis in human hepatoma QGY7703 cells
Author(s) -
Hu Zhenlin,
Guo Xueqing,
Yu Qi,
Qiu Lei,
Li Jianzhong,
Ying Kang,
Guo Cheng,
Zhang Junping
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.12.029
Subject(s) - camptothecin , translocase , apoptosis , mitochondrial permeability transition pore , microbiology and biotechnology , mptp , chemistry , biology , programmed cell death , mitochondrion , biochemistry , gene , chromosomal translocation , dopaminergic , neuroscience , dopamine
Adenine nucleotide translocase (ANT) is known as a core component of the mitochondrial permeability transition pore (MPTP) and a key player in cell death. However, its role in camptothecin (CPT)‐induced apoptosis has not been examined. We showed that CPT‐induced apoptosis in QGY7703 cells and down‐regulated the expression of ANT3. Using ANT3 knock‐out and overexpression experiments, we provide further evidence that ANT3 plays a contributive role in CPT‐induced apoptosis through induction of MPTP. We speculate that the down‐regulation of ANT3 upon stimulation with CPT may be part of the molecular basis underlying the mechanism of acquired resistance to CPT.