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Characterization of chemoreceptive protein binding to an oviposition stimulant using a fluorescent micro‐binding assay in a butterfly
Author(s) -
Tsuchihara Kazuko,
Wazawa Tetsuichi,
Ishii Yoshiharu,
Yanagida Toshio,
Nishida Ritsuo,
Zheng Xiang-Guo,
Ishiguro Masaji,
Yoshihara Kazuo,
Hisatomi Osamu,
Tokunaga Fumio
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.12.027
Subject(s) - aristolochic acid , stimulant , biology , ligand binding assay , ligand (biochemistry) , biochemistry , pharmacology , receptor , genetics
A native female‐specific chemoreceptive protein of a swallowtail butterfly [oviposition stimulant binding protein (OSBP)] was shown to specifically bind to aristolochic acid, a main stimulant for oviposition from its host plant. Oviposition stimulants are recognized by chemoreceptive organs of insects. OSBP isolated previously from the chemoreceptive organs was assumed to bind to an oviposition stimulant. Using a highly sensitive fluorescent micro‐binding assay, we clarified OSBP bound to aristolochic acid. Three‐dimensional molecular modeling revealed the structure of the OSBP‐aristolochic acid complex. This is the first report of a native chemoreceptive protein binding to an oviposition stimulant as a ligand in insects.