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A novel, anaerobically induced ferredoxin in Chlamydomonas reinhardtii
Author(s) -
Jacobs Jessica,
Pudollek Susanne,
Hemschemeier Anja,
Happe Thomas
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.12.018
Subject(s) - chlamydomonas reinhardtii , ferredoxin , chemistry , chlamydomonas , biochemistry , biophysics , biology , enzyme , gene , mutant
We have found the transcript of one of at least six ferredoxin encoding genes of the green alga Chlamydomonas reinhardtii , FDX5 , strongly accumulating in anaerobiosis, indicating a vital role of the encoded protein in the anaerobic metabolism of the cells. According to absorption and electron paramagnetic resonance spectroscopy, Fdx5 is a plant‐type [2Fe2S]‐ferredoxin with a redox potential similar to that of the ferredoxin PetF. However, although Fdx5 seems to be located in the chloroplast, it is not able to photoreduce nicotinamide adenine dinucleotide phosphate (NADP + ) via ferredoxin‐NADP‐reductase, nor to be an electron donor to the plastidic [FeFe]‐hydrogenase HydA1. Thus, Fdx5 seems to have a special role in a yet to be identified anaerobic pathway.