Premium
Silencing efficiency differs among tissues and endogenous microRNA pathway is preserved in short hairpin RNA transgenic mice
Author(s) -
Sasaguri Hiroki,
Mitani Tasuku,
Anzai Masayuki,
Kubodera Takayuki,
Saito Yuki,
Yamada Hiromi,
Mizusawa Hidehiro,
Yokota Takanori
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.12.012
Subject(s) - small hairpin rna , gene silencing , microrna , transgene , biology , genetically modified mouse , endogeny , rna interference , sod1 , gene , microbiology and biotechnology , rna , genetics , biochemistry , mutant
In short hairpin RNA (shRNA) transgenic mice, the tissue difference in gene silencing efficiency and oversaturation of microRNA (miRNA) pathway have not been well assessed. We studied these problems in our previously‐reported anti‐copper/zinc superoxide dismutase (SOD1) shRNA transgenic mice. Although there was a tissue difference (liver and skeletal muscle, >95%; central nervous system and lung, ∼80%), the target gene silencing was systemic and our anti‐SOD1 shRNA transgenic mice recapitulated the SOD1‐null mice. Neither endogenous miRNAs nor their target gene levels were altered, indicating the preservation of endogenous miRNA pathways. We think that the shRNA transgenic mice can be utilized for gene analysis.