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An ISFET biosensor for the monitoring of maltose‐induced conformational changes in MBP
Author(s) -
Park Hye-Jung,
Kim Sang Kyu,
Park Kyoungsook,
Lyu Hong-Kun,
Lee Chang-Soo,
Chung Sang J.,
Yun Wan Soo,
Kim Moonil,
Chung Bong Hyun
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.11.039
Subject(s) - isfet , biosensor , maltose , maltose binding protein , chemistry , field effect transistor , biophysics , substrate (aquarium) , nanotechnology , enzyme , biochemistry , materials science , transistor , fusion protein , biology , recombinant dna , ecology , physics , quantum mechanics , voltage , gene
Here we describe an ion sensitive field effect transistor (ISFET) biosensor, which was designed to monitor directly the surface charge of structurally altered maltose binding protein (MBP) upon stimulation with maltose. This study is the first report of the application of a FET biosensor to the monitoring of conformationally changed proteins. Consequently, a significant drop in current on the basis of the charge‐dependent capacitance measurement has been clearly observed in response to maltose, but not for the glucose control, thereby indicating that the substrate‐specific conformational properties of the target protein could be successfully monitored using the ISFET. Collectively, our results clearly suggest that ISFET provide a high fidelity system for the detection of maltose‐induced structural alterations in MBP.