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Constitutively active GPR6 is located in the intracellular compartments
Author(s) -
Padmanabhan Shalini,
Myers Amanda G.,
Prasad Balakrishna M.
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.11.033
Subject(s) - intracellular , hek 293 cells , microbiology and biotechnology , endogeny , green fluorescent protein , embryonic stem cell , receptor , biology , cell culture , chemistry , biochemistry , gene , genetics
We used multiple imaging assays to test the hypothesis that GPR6, a constitutively active Gs‐coupled receptor, is present on the cell surface. A pHluorin tag at the N‐terminus of rat GPR6 expressed in human embryonic kidney 293 (HEK293) cells was not accessible to protons, chymotrypsin or anti‐green fluorescent protein antibody, demonstrating that GPR6 is primarily located in intracellular compartments. Similar intracellular localization of pHluorin‐tagged GPR6 was found in striatal neurons, where endogenous GPR6 is expressed. Confirmation of Gs‐mediated constitutive activity in HEK293 cells and striatal neurons led us to conclude that GPR6 can signal from intracellular compartments.