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Novel synthetic collagen fibers, poly(PHG), stimulate platelet aggregation through glycoprotein VI
Author(s) -
Inoue Osamu,
Suzuki-Inoue Katsue,
Shinoda Daisuke,
Umeda Yasuto,
Uchino Masazumi,
Takasaki Sin-ichi,
Ozaki Yukio
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.11.026
Subject(s) - gpvi , chemistry , platelet , platelet membrane glycoprotein , collagen receptor , integrin , glycoprotein , platelet adhesiveness , platelet activation , disintegrin , tyrosine phosphorylation , biochemistry , biophysics , phosphorylation , receptor , platelet aggregation , immunology , biology , enzyme , metalloproteinase
Novel synthetic collagen fibers, poly(PHG) made by polycondensation of Pro‐Hyp‐Gly, spontaneously assume polymeric structure with molecular weights greater than 10 5 . Its application for biomaterials has been explored, but that for a platelet agonist has not been investigated. Poly(PHG)‐induced platelet aggregation independently of thromboxane A 2 and integrin α2β1. Poly(PHG)‐induced tyrosine phosphorylation of glycoprotein VI (GPVI)‐related molecules and failed to activate GPVI/FcRγ‐deficient platelets. Binding of GPVI to poly(PHG) was confirmed by a surface plasmon resonance spectroscopy, suggesting that poly(PHG) activates platelets through GPVI. Poly(PHG) is an useful research tool to investigate GPVI‐mediated signals and a substitute for collagen in platelet functional assays.