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AtTIP1;3 and AtTIP5;1 , the only highly expressed Arabidopsis pollen‐specific aquaporins, transport water and urea
Author(s) -
Soto Gabriela,
Alleva Karina,
Mazzella María Agustina,
Amodeo Gabriela,
Muschietti Jorge P.
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.11.002
Subject(s) - aquaporin , arabidopsis , pollen , urea , xenopus , arabidopsis thaliana , glycerol , microbiology and biotechnology , water transport , chemistry , function (biology) , gene , biology , botany , pollination , biochemistry , water flow , mutant , environmental engineering , engineering
Pollination includes processes where water and/or solute movements must be finely regulated, suggesting participation of aquaporins. Using information available from different transcriptional profilings of Arabidopsis thaliana mature pollen, we showed that the only aquaporins that are selectively and highly expressed in mature pollen are two TIPs: AtTIP1;3 and AtTIP5;1 . Pollen exhibited a lower number and more exclusive type of aquaporin expressed genes when compared to other single cell transcriptional profilings. When characterized using Xenopus oocyte swelling assays, At TIP1;3 and At TIP5;1 showed intermediate water permeabilities. Although they displayed neither glycerol nor boric acid permeability they both transported urea. In conclusion, these results suggest a function for At TIP1;3 and At TIP5;1 as specific water and urea channels in Arabidopsis pollen.