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A strategy for screening an inhibitor of viral silencing suppressors, which attenuates symptom development of plant viruses
Author(s) -
Shimura Hanako,
Fukagawa Takako,
Meguro Ayano,
Yamada Hirokazu,
Oh-hira Mahito,
Sano Shinsuke,
Masuta Chikara
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.10.046
Subject(s) - gene silencing , surface plasmon resonance , small interfering rna , virology , rna interference , biology , in vivo , rna silencing , virus , chemistry , rna , biochemistry , microbiology and biotechnology , gene , nanotechnology , materials science , nanoparticle
To find out whether we can control plant virus diseases by blocking viral RNA silencing suppressors (RSSs), we developed a strategy to screen inhibitors that block the association of RSSs with siRNAs using a surface plasmon resonance assay. The screened chemicals were tested in competition with RSSs for binding to siRNAs using a mobility shift assay. We then confirmed that tested chemicals actually inhibited the RSS activity in vivo using a protoplast assay which was developed for this purpose. This entire system can be adapted to screening inhibitors of not only plant viruses but also some animal viruses possessing RSSs.