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Reconstruction of a bacterial isoprenoid biosynthetic pathway in Saccharomyces cerevisiae
Author(s) -
Maury Jérôme,
Asadollahi Mohammad A.,
Møller Kasper,
Schalk Michel,
Clark Anthony,
Formenti Luca R.,
Nielsen Jens
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.10.045
Subject(s) - saccharomyces cerevisiae , mevalonate pathway , yeast , escherichia coli , biochemistry , metabolic pathway , biology , terpenoid , gene , biosynthesis , enzyme , model organism , metabolic engineering
A eukaryotic mevalonate pathway transferred and expressed in Escherichia coli, and a mammalian hydrocortisone biosynthetic pathway rebuilt in Saccharomyces cerevisiae are examples showing that transferring metabolic pathways from one organism to another can have a powerful impact on cell properties. In this study, we reconstructed the E. coli isoprenoid biosynthetic pathway in S. cerevisiae . Genes encoding the seven enzymatic steps of the pathway were cloned and expressed in S. cerevisiae . mRNA from the seven genes was detected, and the pathway was shown able to sustain growth of yeast in conditions of inhibition of its constitutive isoprenoid biosynthetic pathway.