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X‐ray structure of the metal‐sensor CnrX in both the apo‐ and copper‐bound forms
Author(s) -
Pompidor Guillaume,
Maillard Antoine P.,
Girard Eric,
Gambarelli Serge,
Kahn Richard,
Covès Jacques
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.10.042
Subject(s) - copper , crystallography , metal , chemistry , ligand (biochemistry) , helix (gastropod) , stereochemistry , receptor , biology , biochemistry , organic chemistry , ecology , snail
Both the X‐ray structures of the apo ‐ and the copper‐bound forms of the metal‐sensor domain (residues 31–148) of CnrX from Cupriavidus metallidurans CH34 were obtained at 1.74 Å resolution from a selenomethionine derivative. This four‐helix hooked‐hairpin is the first structure of a metal‐sensor in an ECF‐type signaling pathway. The copper ion is bound in a type 2‐like center with a 3N1O coordination in the equatorial plane and shows an unprecedented remote fifth axial ligand with Met93 contributing a weak S–Cu bond. The signal onset cannot be explained by conformational changes associated with CnrX metallation.