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Specific interaction of TFII‐I with an upstream element on the HIV‐1 LTR regulates induction of latent provirus
Author(s) -
Malcolm Tom,
Kam Joanna,
Pour Pouya Sadeghi,
Sadowski Ivan
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.10.032
Subject(s) - provirus , response element , biology , hiv long terminal repeat , microbiology and biotechnology , virology , long terminal repeat , physics , genetics , gene , promoter , gene expression , genome
RBF‐2 is a factor comprised of a USF1/2 heterodimer, whose association with a highly conserved upstream element (RBEIII) on the HIV‐1 LTR requires a co‐factor TFII‐I. We have identified specific nucleotides, immediately 3′ of RBEIII that are required for stable association of TFII‐I with this region of the LTR. Mutations that inhibit interaction of TFII‐I with DNA also prevent stimulation of USF binding to RBEIII, and render the integrated LTR unresponsive to T cell signaling. These results demonstrate an essential role of TFII‐I bound at an upstream LTR element for viral replication.