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Nucleophosmin is required for chromosome congression, proper mitotic spindle formation, and kinetochore‐microtubule attachment in HeLa cells
Author(s) -
Amin Mohammed Abdullahel,
Matsunaga Sachihiro,
Uchiyama Susumu,
Fukui Kiichi
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.10.023
Subject(s) - mitosis , microbiology and biotechnology , kinetochore , nucleophosmin , mitotic exit , centrosome , multipolar spindles , spindle checkpoint , spindle apparatus , biology , plk1 , cell cycle checkpoint , mitotic catastrophe , microtubule , chemistry , cell division , cell cycle , cancer research , cell , chromosome , genetics , myeloid leukemia , gene
Nucleophosmin (NPM) is an abundantly expressed multifunctional nucleolar phosphoprotein. Here we show that depletion of NPM by RNA interference causes defects in cell division, followed by an arrest of DNA synthesis due to activation of a p53‐dependent checkpoint response in HeLa cells. Depletion of NPM leads to mitotic arrest due to spindle checkpoint activation. The mitotic cells arrested by NPM depletion have defects in chromosome congression, proper mitotic spindle and centrosome formation, as well as defects in kinetochore‐microtubule attachments. Loss of NPM thus causes severe mitotic defects and delayed mitotic progression. These findings indicate that NPM is essential for mitotic progression and cell proliferation.