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Reciprocal influences of CB 1 cannabinoid receptor agonists on ERK and JNK signalling in N1E‐115 cells
Author(s) -
Bosier Barbara,
Lambert Didier M.,
Hermans Emmanuel
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.10.022
Subject(s) - mapk/erk pathway , cannabinoid receptor , kinase , phosphorylation , signalling , cannabinoid , microbiology and biotechnology , receptor , chemistry , biology , biochemistry , agonist
Agonists acting at the CB 1 cannabinoid receptor in N1E‐115 neuroblastoma cells were found to activate MAPK family members with reciprocal efficacies. Thus, HU 210 robustly increased phosphorylation of ERK1/2 whereas CP 55,940 was more effective in activating JNK. The use of selected kinase inhibitors confirmed that distinct signalling cascades were involved in these responses. This reciprocal control of MAPK activity was correlated with the observation that HU 210‐ and CP 55,940‐mediated regulations of tyrosine hydroxylase gene expression were respectively impaired by MEK and JNK inhibitors. These data indicate that complex interactions of the CB 1 receptor with intracellular signalling partners controlling MAPK activities may explain the apparent disparities in cellular responses to functional selective agonists.