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Inhibitory effects of gallic acid and quercetin on UDP‐glucose dehydrogenase activity
Author(s) -
Hwang Eun Young,
Huh Jae-Wan,
Choi Myung-Min,
Choi Soo Young,
Hong Hea-Nam,
Cho Sung-Woo
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.10.010
Subject(s) - gallic acid , quercetin , chemistry , biochemistry , lactate dehydrogenase , dehydrogenase , enzyme , antioxidant
We have examined polyphenols as potential inhibitors of UDP‐glucose dehydrogenase (UGDH) activity. Gallic acid and quercetin decreased specific activities of UGDH and inhibited the proliferation of MCF‐7 human breast cancer cells. Western blot analysis showed that gallic acid and quercetin did not affect UGDH protein expression, suggesting that UGDH activity is inhibited by polyphenols at the post‐translational level. Kinetics studies using human UGDH revealed that gallic acid was a non‐competitive inhibitor with respect to UDP‐glucose and NAD + . In contrast, quercetin showed a competitive inhibition and a mixed‐type inhibition with respect to UDP‐glucose and NAD + , respectively. These results indicate that gallic acid and quercetin are effective inhibitors of UGDH that exert strong antiproliferative activity in breast cancer cells.