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Brief cross‐linking of Fas/APO‐1 (CD95) triggers engulfment of pre‐apoptotic target cells
Author(s) -
Zhang Shouting,
Witasp Erika,
Lauwen Marjolein,
Fadeel Bengt
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.09.020
Subject(s) - jurkat cells , apoptosis , fas receptor , microbiology and biotechnology , tumor necrosis factor alpha , macrophage , phagocytosis , annexin , fas ligand , receptor , chemistry , biology , immune system , immunology , programmed cell death , t cell , biochemistry , in vitro
Macrophage clearance of dying cells is of crucial importance to maintain tissue homeostasis. Here, we show that brief treatment (15 min) of Jurkat T cells with agonistic anti‐Fas antibodies or recombinant Fas ligand results in efficient phagocytosis by human monocyte‐derived macrophages prior to the occurrence of common biomarkers of apoptosis. Similar findings were obtained when using primary human T cells. Macrophage engulfment of pre‐apoptotic target cells was suppressed in the absence of serum. Moreover, pre‐apoptotic cells secreted annexin I and administration of Boc1, a formyl peptide receptor/lipoxin receptor antagonist markedly attenuated their engulfment. Finally, pre‐apoptotic Jurkat cells induced lower macrophage production of tumor necrosis factor‐α and higher production of interleukin‐10 in comparison to apoptotic target cells.