Premium
Nuclear matrix contains novel WD‐repeat and disordered‐region‐rich proteins
Author(s) -
Ishii Kohei,
Hirano Yasuhiro,
Araki Nanase,
Oda Toshiyuki,
Kumeta Masahiro,
Takeyasu Kunio,
Furukawa Kazuhiro,
Horigome Tsuneyoshi
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.09.019
Subject(s) - nuclear matrix , proteome , chemistry , nuclear protein , biochemistry , dna , transcription factor , chromatin , gene
To find novel proteins predicted to participate in the formation of nuclear bodies, nuclear speckles, and nuclear macro‐protein complexes, we applied proteome analysis to a HeLa cell nuclear matrix fraction. Proteins in the fraction were separated by SDS–PAGE, digested with trypsin, and analyzed by nanoflow liquid chromatography–iontrap‐tandem mass spectrometry. Three hundred and thirty three proteins including 39 novel ones were identified. Seven WD‐repeat proteins and 16 disordered region‐rich proteins, which act frequently as scaffolding proteins for macro‐protein complexes, were found amongst the novel proteins.