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GO6976 prevents TNF‐α‐induced suppression of adiponectin expression in 3T3‐L1 adipocytes: Putative involvement of protein kinase C
Author(s) -
Lim Joong-Yeon,
Kim Won Ho,
Park Sang Ick
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.09.012
Subject(s) - adiponectin , adipocyte , adipokine , 3t3 l1 , endocrinology , medicine , tumor necrosis factor alpha , protein kinase a , protein kinase c , secretion , biology , kinase , chemistry , microbiology and biotechnology , adipose tissue , insulin , insulin resistance
Adiponectin, one of the adipokines secreted by adipocytes, possesses insulin sensitizing and anti‐atherosclerotic properties. Tumor necrosis factor‐alpha (TNF‐α) is known to suppress the expression and secretion of adiponectin in adipocytes; however, the underlying mechanism remains poorly understood. Here, we demonstrate that GO6976 (a selective inhibitor of conventional protein kinase C (PKC)) prevents TNF‐α‐induced suppression of adiponectin secretion and expression in fully differentiated 3T3‐L1 adipocytes, accompanied by attenuation of c‐Jun N‐terminal kinase (JNK) activation. Additionally, the transcriptional activity of peroxisome proliferator‐activated receptor‐gamma (PPARg) (a strong inducer of adiponectin) on the adiponectin promoter was inhibited in a PKC isoform‐specific manner. These results raise the possibility that PKC is involved in TNF‐α‐induced suppression of adiponectin in 3T3‐L1 adipocytes.