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Stress‐induced down‐regulation of tumor‐associated NADH oxidase during apoptosis in transformed cells
Author(s) -
Mao Liang-Chi,
Wang Hsi-Ming,
Lin You-Yu,
Chang Te-Kau,
Hsin Yi-Hong,
Chueh Pin Ju
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.09.008
Subject(s) - gene knockdown , apoptosis , cell growth , hela , microbiology and biotechnology , rna interference , small hairpin rna , cell , transfection , cancer cell , biology , cancer research , cell culture , cancer , rna , biochemistry , gene , genetics
Tumor‐associated NADH oxidase (tNOX) is a growth‐related protein expressed in transformed cells. tNOX knockdown using RNA interference leads to a significant reduction in HeLa cell proliferation and migration, indicating an important role for tNOX in growth regulation and the cancer phenotype. Here, we show that tNOX is down‐regulated during apoptosis in HCT116 cells. Treatment with diverse stresses induced a dose‐ and time‐dependent decrease in tNOX expression that was concurrent with apoptosis. Moreover, shRNA‐mediated tNOX knockdown rendered cells susceptible to apoptosis, whereas re‐expression of tNOX partially recovered cell proliferation. Our results indicate that tNOX is suppressed during apoptosis and demonstrate that tNOX down‐regulation sensitizes cells to stress‐induced growth reduction, suggesting that tNOX is required for transformed cell growth.