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Importance of C‐terminal flexible region of 4E‐binding protein in binding with eukaryotic initiation factor 4E
Author(s) -
Mizuno Atsuo,
In Yasuko,
Fujita Yuki,
Abiko Fumi,
Miyagawa Hiroo,
Kitamura Kunihiro,
Tomoo Koji,
Ishida Toshimasa
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.09.003
Subject(s) - eif4e , binding site , terminal (telecommunication) , plasma protein binding , binding protein , surface plasmon resonance , biophysics , biology , microbiology and biotechnology , biochemistry , materials science , gene , nanotechnology , messenger rna , translation (biology) , computer science , telecommunications , nanoparticle
Although the α‐helical Y(X) 4 Lϕ containing region of eIF4E‐binding protein (4EBP) is the major binding region with eukaryotic initiation factor 4E (eIF4E), the roles of its N‐ and C‐terminal regions in the binding are hardly known. To clarify the roles of these flexible regions in the interaction, the binding features of the sequentially N‐, C‐, or both‐terminal‐residue‐deleted 4EBP2 mutants were investigated by surface plasmon resonance (SPR) analysis. It was shown that the C‐terminal His74‐Glu89 sequence has an auxiliary, but indispensable, function in stabilizing the binding to eIF4E. The possible interaction with eIF4E was estimated by molecular dynamics simulation. This is the first report on the importance of the C‐terminal flexible region in the eIF4E‐binding regulation of 4EBP.