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Study of the interaction of the Ig2 module of the fibroblast growth factor receptor, FGFR Ig2, with the fibroblast growth factor 1, FGF1, by means of NMR spectroscopy
Author(s) -
Kochoyan Artur,
Poulsen Flemming M.,
Berezin Vladimir,
Bock Elisabeth,
Kiselyov Vladislav V.
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.08.033
Subject(s) - fgf1 , fibroblast growth factor receptor , fibroblast growth factor , fibroblast growth factor receptor 1 , fibroblast growth factor receptor 2 , fibroblast growth factor receptor 3 , fibroblast growth factor receptor 4 , chemistry , fibroblast , microbiology and biotechnology , receptor , biology , biochemistry , in vitro
Fibroblast growth factor (FGF) receptor (FGFR) consists extracellularly of three immunoglobulin (Ig) modules (Ig1–3). Currently, there are two competing models (symmetric and asymmetric) of the FGF–FGFR–heparin complex based on crystal structures. Indirect evidence exists in support of both models. However, it is not clear which model is physiologically relevant. Our aim was to obtain direct, non‐crystallographic evidence in support of them. We found by nuclear magnetic resonance that Ig2 could bind to FGF1 not only via the primary site (present in both models), but also via the secondary site (present only in the symmetric model). Thus, our data support the symmetric model.