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hOLFML1, a novel secreted glycoprotein, enhances the proliferation of human cancer cell lines in vitro
Author(s) -
Wan Bingbing,
Zhou Yu-Bo,
Zhang Xin,
Zhu Hong,
Huo Keke,
Han Ze-Guang
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.08.009
Subject(s) - glycoprotein , in vitro , cell growth , secretion , microbiology and biotechnology , immunohistochemistry , cell culture , secretory protein , biology , hela , cell , chemistry , biochemistry , immunology , genetics
We describe a novel secreted protein, named hOLFML1 (human olfactomedin‐like protein 1), with an olfectamine domain in its C‐terminus, mainly expressed in the small intestine, liver, lung and heart. Immunohistochemical staining on human small intestine indicated that the protein localizes preferentially in the intestinal villi. Interestingly, ectopic hOLFML1 promoted proliferation of HeLa cells and increased the percentage of cells in S phase. In contrast, knock down of hOLFML1 protein expression by siRNA inhibited cell proliferation and delayed the entry of cells into S phase. Our data also revealed that hOLFML1 is N ‐glycosylated and its secretion is triggered by serum. Taken together, these findings suggest that hOLFML1 may play a significant role in the regulation of cell proliferation in vitro.