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Cyclophilin‐40 has a cellular role in the aryl hydrocarbon receptor signaling
Author(s) -
Luu Tony C.,
Bhattacharya Pompeya,
Chan William K.
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.08.007
Subject(s) - aryl hydrocarbon receptor , cyclophilin , cyclophilin a , chemistry , microbiology and biotechnology , signal transduction , receptor , biochemistry , biology , transcription factor , gene
Cyclophilin‐40 (CyP40) promotes the formation of the gel shift complex that contains the aryl hydrocarbon receptor (AhR), AhR nuclear translocator (Arnt) and dioxin response element (DRE) using baculovirus expressed proteins. Here we reported that CyP40 plays a role in the AhR signaling. When the CyP40 content in MCF‐7 cells is reduced, up‐regulation of cyp1a1 and cyp1b1 by 3‐methylchloranthrene (3MC) is also reduced, suggesting that CyP40 is essential for maximal AhR function. The CyP40 region containing amino acids 186–215, but not the peptidyl‐prolyl cis – trans isomerase and tetratricopeptide repeat domains, is essential for forming the AhR/Arnt/DRE complex. CyP40 is found in the cell nucleus after 3MC treatment and appears to promote the DRE binding form of the AhR/Arnt heterodimer.