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Type II NAD(P)H dehydrogenases are targeted to mitochondria and chloroplasts or peroxisomes in Arabidopsis thaliana
Author(s) -
Carrie Chris,
Murcha Monika W.,
Kuehn Kristina,
Duncan Owen,
Barthet Michelle,
Smith Penelope M.,
Eubel Holger,
Meyer Etienne,
Day David A.,
Millar A. Harvey,
Whelan James
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.07.061
Subject(s) - peroxisome , chloroplast , mitochondrion , organelle , arabidopsis thaliana , nad+ kinase , biochemistry , protein targeting , biology , plastid , arabidopsis , peroxisomal targeting signal , microbiology and biotechnology , enzyme , membrane protein , gene , membrane , mutant
We found that four type II NAD(P)H dehydrogenases (ND) in Arabidopsis are targeted to two locations in the cell; NDC1 was targeted to mitochondria and chloroplasts, while NDA1, NDA2 and NDB1 were targeted to mitochondria and peroxisomes. Targeting of NDC1 to chloroplasts as well as mitochondria was shown using in vitro and in vivo uptake assays and dual targeting of NDC1 to plastids relies on regions in the mature part of the protein. Accumulation of NDA type dehydrogenases to peroxisomes and mitochondria was confirmed using Western blot analysis on highly purified organelle fractions. Targeting of ND proteins to mitochondria and peroxisomes is achieved by two separate signals, a C‐terminal signal for peroxisomes and an N‐terminal signal for mitochondria.