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Suppression of hepatitis C virus replication by baculovirus vector‐mediated short‐hairpin RNA expression
Author(s) -
Suzuki Hitoshi,
Tamai Nobushige,
Habu Yuichiro,
Chang Myint O.O.,
Takaku Hiroshi
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.07.056
Subject(s) - virology , vector (molecular biology) , small hairpin rna , rna , viral replication , biology , hepatitis a virus , replication (statistics) , virus , genetics , gene , recombinant dna
Short‐hairpin RNAs (shRNAs) inhibit gene expression by RNA interference. Here, we report on the inhibition, by baculovirus‐based vector‐derived shRNAs, of core‐protein expression in full‐length hepatitis C virus (HCV) replicon cells. shRNAs were designed to target the highly conserved core region of the HCV genome. In particular, the core‐shRNA452 containing nucleotides 452–472, as the target in the HCV core gene, dramatically inhibited the expression of the HCV core protein in replicon cells. Furthermore, HCV core‐protein expression was inhibited more strongly by the vesicular stomatitis virus glycoprotein (VSV‐G)‐pseudotyped baculovirus vector than by the wild‐type baculovirus vector.