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l ‐2,3‐Diaminopropionate: One of the building blocks for the biosynthesis of Zwittermicin A in Bacillus thuringiensis subsp. kurstaki strain YBT‐1520
Author(s) -
Zhao Changming,
Song Chunxu,
Luo Yi,
Yu Ziniu,
Sun Ming
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.07.054
Subject(s) - bacillus thuringiensis , strain (injury) , biosynthesis , chemistry , microbiology and biotechnology , biology , bacteria , biochemistry , gene , genetics , anatomy
Zwittermicin A (ZwA) is a hybrid polyketide–non‐ribosomal peptide that is thought to be biosynthesized from five proposed building blocks, including the 2,3‐diaminopropionate. Candidate genes for de novo biosynthesis of 2,3‐diaminopropionate, zwa5A and zwa5B , have been identified in a previous study. In this research, zwa5A was interrupted and chemically synthesized 2,3‐diaminopropionate was used to feed the zwa5A − mutant. Results showed that feeding with 2,3‐diaminopropionate restored the ability of the zwa5A − mutant to produce ZwA. Another non‐ribosomal peptide synthase gene, designated orf3 , was identified. Amino acid dependent PPi release assay showed that the adenylation domain ZWAA2 of ORF3 acyl‐adenylated l ‐2,3‐diaminopropionate effectively. Taken together, it can be concluded that l ‐2,3‐diaminopropionate is indeed one of the building blocks for the biosynthesis of Zwittermicin A.