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A halorhodopsin‐overproducing mutant isolated from an extremely haloalkaliphilic archaeon Natronomonas pharaonis
Author(s) -
Ihara Kunio,
Narusawa Akihiro,
Maruyama Keita,
Takeguchi Masashi,
Kouyama Tsutomu
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.07.030
Subject(s) - mutant , halorhodopsin , halobacteriaceae , biology , biochemistry , reversion , strain (injury) , bacteriorhodopsin , activator (genetics) , membrane , wild type , photoprotein , microbiology and biotechnology , halobacterium salinarum , chemistry , gene , bioluminescence , phenotype , anatomy
The halorhodopsin (hR)‐overproducing mutant strain KM‐1 was isolated from the extremely haloalkaliphilic archaeon Natronomonas pharaonis type strain DSM2160 T . hR‐enriched membranes were easily obtained by washing the cells with distilled water. The membranes were claret colored owing to two pigments: hR and bacterioruberin. The hR component in the absorption spectra changed from blue to purple upon the addition of Cl − and had a K m value of 1.7 mM. Overexpression of hR in strain KM‐1 might be caused by the point mutation Asp324 → Asn in the bacteriorhodopsin activator homologues of N. pharaonis . The mutation changed the hR‐expression pattern from inducible to constitutive in the late exponential phase.