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Metabolomic profiling of Drosophila using liquid chromatography Fourier transform mass spectrometry
Author(s) -
Kamleh M.A.,
Hobani Y.,
Dow J.A.T.,
Watson D.G.
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.07.029
Subject(s) - metabolomics , chemistry , hypoxanthine , chromatography , xanthine , hydrophilic interaction chromatography , mass spectrometry , allantoin , orbitrap , glycerophospholipid , metabolic pathway , pyrimidine metabolism , biochemistry , metabolism , high performance liquid chromatography , enzyme , phospholipid , purine , membrane
Hydrophilic interaction chromatography (HILIC) interfaced with an Orbitrap Fourier transform mass spectrometer (FT‐MS) was used to carry out metabolomic profiling of the classical Drosophila mutation, rosy ( ry ). This gene encodes a xanthine oxidase/dehydrogenase. In addition to validating the technology by detecting the same changes in xanthine, hypoxanthine, urate and allantoin that have been reported classically, completely unsuspected changes were detected in each of the tryptophan, arginine, pyrimidine and glycerophospholipid metabolism pathways. The rosy mutation thus ramifies far more widely than previously detected.