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NADPH‐dependent thioredoxin reductase and 2‐Cys peroxiredoxins are needed for the protection of Mg–protoporphyrin monomethyl ester cyclase
Author(s) -
Stenbaek Anne,
Hansson Andreas,
Wulff Ragna Peterson,
Hansson Mats,
Dietz Karl-Josef,
Jensen Poul Erik
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.07.006
Subject(s) - protochlorophyllide , chloroplast , thioredoxin reductase , biochemistry , hydrogen peroxide , peroxiredoxin , chemistry , cyclase , thioredoxin , biosynthesis , enzyme , peroxidase , gene
The chloroplast‐localized NADPH‐dependent thioredoxin reductase (NTRC) has been found to be able to reduce hydrogen peroxide scavenging 2‐Cys peroxiredoxins. We show that the Arabidopsis ntrc mutant is perturbed in chlorophyll biosynthesis and accumulate intermediates preceding protochlorophyllide formation. A specific involvement of NTRC during biosynthesis of protochlorophyllide is indicated from in vitro aerobic cyclase assays in which the conversion of Mg–protoporhyrin monomethyl ester into protochlorophyllide is stimulated by addition of the NTRC/2‐Cys peroxiredoxin system. These findings support the hypothesis that this NADPH‐dependent hydrogen peroxide scavenging system is particularly important during periods with limited reducing power from photosynthesis, e.g . under chloroplast biogenesis.