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Dehydroascorbate and glucose are taken up into Arabidopsis thaliana cell cultures by two distinct mechanisms
Author(s) -
Horemans N.,
Szarka A.,
De Bock M.,
Raeymaekers T.,
Potters G.,
Levine M.,
Banhégyi G.,
Guisez Y.
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.07.001
Subject(s) - phloretin , arabidopsis thaliana , cytochalasin b , plant cell , phlorizin , biochemistry , chemistry , glucose transporter , glucose uptake , transporter , arabidopsis , cell , biology , gene , insulin , endocrinology , mutant
The possible involvement of glucose (Glc) carriers in the uptake of vitamin C in plant cells is still a matter of debate. For the first time, it was shown here that plant cells exclusively take up the oxidised dehydroascorbate (DHA) form. DHA uptake is not affected by 6‐bromo‐6‐deoxy‐ascorbate, an ascorbate (ASC) analogue, specifically demonstrating ASC uptake in animal cells. There is no competition between Glc and DHA uptake. Moreover, DHA and Glc carriers respond in the opposite manner to different inhibitors (cytochalasin B, phloretin and genistein). In conclusion, the plant plasma membrane DHA carrier is distinct from the plant Glc transporters.