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Identification of a rare subset of adipose tissue‐resident progenitor cells, which express CD133 and TRPC3 as a VEGF‐regulated Ca 2+ entry channel
Author(s) -
Poteser Michael,
Graziani Annarita,
Eder Petra,
Yates Ameli,
Mächler Heinrich,
Romanin Christoph,
Groschner Klaus
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.06.049
Subject(s) - trpc3 , progenitor cell , identification (biology) , adipose tissue , microbiology and biotechnology , chemistry , biology , stem cell , biochemistry , trpc , botany , receptor , transient receptor potential channel
VEGF‐induced Ca 2+ signalling was investigated in CD133 + /VEGFR‐2 + progenitor cells isolated from human adipose stroma. Colonies derived from CD133 + immunoselected cells displayed inhomogenous Ca 2+ signals, with variable magnitude of VEGF‐induced Ca 2+ entry, which positively correlated with expression of the Ca 2+ channel protein TRPC3. High levels of VEGF‐induced Ca 2+ entry and TRPC3 expression were preferentially detected in rim areas of expanding colonies. Dominant negative suppression of TRPC3 inhibited VEGF‐induced Ca 2+ entry into CD133 + cells. Our results identify TRPC3 as a key Ca 2+ entry channel in a subset of CD133 + stem cells. We suggest TRPC3 as an essential determinant of cell fate in CD133 + progenitor‐derived colonies.