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Abrogation of G2/M arrest sensitizes curcumin‐resistant hepatoma cells to apoptosis
Author(s) -
Wang Wei-Zhang,
Cheng Jiasen,
Luo Jing,
Zhuang Shi-Mei
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.06.048
Subject(s) - curcumin , apoptosis , downregulation and upregulation , cell cycle checkpoint , chek1 , microbiology and biotechnology , chemistry , cancer research , biology , cell cycle , biochemistry , gene
In this study, we showed that curcumin treatment resulted in activation of Chk1‐mediated G2 checkpoint, which was associated with the induction of G2/M arrest and the resistance of cancer cells to curcumin‐induced apoptosis. Further investigation revealed that inhibition of Chk1 significantly abrogated G2/M arrest and sensitized curcumin‐resistant cells to apoptosis via upregulation of Bad and in turn the loss of mitochondrial membrane potential. These results indicate that Chk1‐mediated G2/M arrest may serve as a mechanism for curcumin resistance and Chk1 represents a potential target for the reversal of this resistance. Our findings should be helpful for clinical application of curcumin.