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Metabolic profiling reveals disorder of amino acid metabolism in four brain regions from a rat model of chronic unpredictable mild stress
Author(s) -
Ni Yan,
Su Mingming,
Lin Jinchao,
Wang Xiaoyan,
Qiu Yunping,
Zhao Aihua,
Chen Tianlu,
Jia Wei
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.06.040
Subject(s) - neurochemical , chronic stress , thalamus , amino acid metabolism , neuroscience , amino acid , profiling (computer programming) , metabolism , biology , bioinformatics , medicine , endocrinology , biochemistry , computer science , operating system
Chronic stress is closely linked to clinical depression, which could be assessed by a chronic unpredictable mild stress (CUMS) animal model. We present here a GC/MS‐based metabolic profiling approach to investigate neurochemical changes in the cerebral cortex, hippocampus, thalamus, and remaining brain tissues. Multi‐criteria assessment for multivariate statistics could identify differential metabolites between the CUMS‐model rats versus the healthy controls. This study demonstrates that the significantly perturbed metabolites mainly involving amino acids play an indispensable role in regulating neural activity in the brain. Therefore, results obtained from such metabolic profiling strategy potentially provide a unique perspective on molecular mechanisms of chronic stress.