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Endoplasmic reticulum stress response in murine kidney exposed to acute hypobaric hypoxia
Author(s) -
Karar Jayashree,
Dolt Karamjit S.,
Qadar Pasha M.A.
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.06.019
Subject(s) - xbp1 , endoplasmic reticulum , unfolded protein response , messenger rna , rna splicing , proteostasis , transcription (linguistics) , microbiology and biotechnology , hypoxia (environmental) , transcription factor , biology , chemistry , medicine , endocrinology , gene , rna , biochemistry , linguistics , philosophy , organic chemistry , oxygen
Any perturbation in the normal functioning of endoplasmic reticulum (ER), such as due to hypoxia, triggers the unfolded protein response (UPR). We studied the temporal variation in gene expression in murine kidney exposed to acute hypobaric hypoxia. Molecular chaperones like Grp78, Grp94, Canx and Calr in the ER were transcriptionally downregulated. Further, the splicing of Xbp1 mRNA decreased, whereas transcription of the unspliced mRNA increased. This step produces Xbp1 protein, which is negatively regulated by the unspliced protein. Hence, the decreased splicing of Xbp1 along with decreased transcription of ER chaperones in kidney is a definite indication of reduced stress.