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A Synechocystis LexA‐orthologue binds direct repeats in target genes
Author(s) -
Patterson-Fortin Laura M.,
Owttrim George W.
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.06.009
Subject(s) - repressor lexa , regulon , biology , synechocystis , gene , genetics , consensus sequence , repressor , regulation of gene expression , peptide sequence , gene expression , mutant
Although evidence for LexA‐orthologues, which do not regulate DNA damage repair, is accumulating, identification of binding sites and regulon members remains poorly characterized. In the cyanobacterium, Synechocystis sp. strain PCC 6803, we have recently identified a LexA‐related protein that regulates expression of the crhR RNA helicase gene. Here we show that the Synechocystis LexA‐orthologue binds as a dimer to 12 bp direct repeats containing a CTA‐N9‐CTA sequence conserved in two target genes, lexA and crhR . Characterization of this site provides the basis for identification of additional LexA targets and further evidence for LexA's divergence during evolution.