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TBP recruitment to the U1 snRNA gene promoter is disrupted by substituting a U6 proximal sequence element A (PSEA) for the U1 PSEA
Author(s) -
Barakat Nermeen H.,
Stumph William E.
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.06.003
Subject(s) - transcription (linguistics) , biology , rna , small nuclear rna , gene , chromatin immunoprecipitation , microbiology and biotechnology , genetics , gene expression , promoter , rna dependent rna polymerase , philosophy , linguistics
Transcription of Drosophila U1 or U6 snRNAs by RNA polymerases II and III respectively requires a unique ∼21 base‐pair promoter element termed the proximal sequence element A (PSEA) recognized by the snRNA activating protein complex (DmSNAPc). A five‐nucleotide substitution that changed the U1 PSEA to a U6 PSEA inactivated the U1 promoter. Chromatin immunoprecipitation assays indicated this substitution did not affect DmSNAPc DNA binding but instead interfered with SNAPc recruitment of TBP to the TATA‐less U1 promoter. These findings support a model wherein sequence differences between the U1 and U6 PSEAs induce distinct DmSNAPc conformational states involved in RNA polymerase selectivity.