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Gramicidin S identified as a potent inhibitor for cytochrome bd ‐type quinol oxidase
Author(s) -
Mogi Tatsushi,
Ui Hideaki,
Shiomi Kazuro,
Ōmura Satoshi,
Kita Kiyoshi
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.05.031
Subject(s) - gramicidin s , gramicidin , chemistry , paracoccus denitrificans , biochemistry , escherichia coli , cytochrome , antibiotics , oxidase test , stereochemistry , enzyme , membrane , gene
Gramicidin S, a cationic cyclic decapeptide, exhibits the potent antibiotic activity through perturbation of lipid bilayers of the bacterial membrane. From the screening of natural antibiotics, we identified gramicidin S as a potent inhibitor for cytochrome bd ‐type quinol oxidase from Escherichia coli . We found that gramicidin S inhibited the oxidase with IC 50 of 3.5 μM by decreasing V max and the affinity for substrates but showed the stimulatory effect at low concentrations. Our findings would provide a new insight into the development of gramicidin S analogs, which do not share the target and mechanism with conventional antibiotics.