Premium
Dcytb (Cybrd1) functions as both a ferric and a cupric reductase in vitro
Author(s) -
Wyman Steven,
Simpson Robert J.,
McKie Andrew T.,
Sharp Paul A.
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.05.010
Subject(s) - in vitro , chemistry , ferric , biochemistry , reductase , enzyme , inorganic chemistry
MDCK cells expressing an inducible duodenal cytochrome b ‐green fluorescent protein (Dcytb‐EGFP) fusion construct were used to investigate the function of Dcytb. The Dcytb‐EGFP protein was targeted correctly to the plasma membrane, and cells displayed increased ferric and cupric reductase activities, which were greatly reduced in the presence of doxycycline. The data suggests that Dcytb plays a physiological role in both iron and copper uptake, through divalent metal transporter 1 (DMT1) and copper transporter 1, respectively. In support of this hypothesis, we show that 59 Fe uptake was significantly enhanced in Dcytb‐EGFP expressing MDCK cells which endogenously express DMT1.