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Globin‐coupled sensors and protoglobins share a common signaling mechanism
Author(s) -
Saito Jennifer A.,
Wan Xuehua,
Lee Kit Shan,
Hou Shaobin,
Alam Maqsudul
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.05.004
Subject(s) - mechanism (biology) , globin , chemistry , microbiology and biotechnology , signal transduction , biophysics , computational biology , biochemistry , biology , hemoglobin , physics , quantum mechanics
The globin‐coupled sensors (GCSs) and protoglobins (Pgbs) form one lineage of the globin superfamily. The GCSs are multidomain sensory proteins involved in aerotaxis or gene regulation, while the Pgbs are single‐domain globins of yet unknown function. We postulate that the GCSs and Pgbs share a common signaling mechanism to modulate diverse physiological functions. To elucidate the signaling properties of individual globin domains, we constructed and expressed chimeric receptors in Escherichia coli . We demonstrate that all the chimeric receptors reversibly bind oxygen in vitro and trigger aerotactic responses in vivo. Thus, oxygen binding to the globin domains of diverse GCSs and Pgbs form a common signaling state that can trigger aerotactic responses.