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Glutamate‐induced glioma cell proliferation is prevented by functional expression of the glutamate transporter GLT‐1
Author(s) -
Vanhoutte Nicolas,
Hermans Emmanuel
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.04.053
Subject(s) - glutamate receptor , cystine , glioma , transporter , chemistry , microbiology and biotechnology , glutamate aspartate transporter , biochemistry , biology , excitatory amino acid transporter , cancer research , gene , enzyme , receptor , cysteine
A tetracycline‐dependent inducible system was used to achieve controlled expression of the glutamate transporter 1 (GLT‐1) in C6 glioma cells. Non‐induced cells show modest glutamate uptake and, in the presence of l ‐cystine, these cells tend to release substantial amounts of glutamate. Overnight exposure to doxycycline increased d ‐[ 3 H]‐aspartate uptake, reaching similar capacity as observed in cultured astrocytes. Efficient clearance of exogenously applied glutamate was evidenced in these cells, even in the presence of l ‐cystine. The addition of glutamate (100 μM) to the medium of non‐induced cells significantly increased their proliferation rate, an effect that was blocked when the expression of GLT‐1 was induced. This suggests that impaired glutamate uptake capacity in glioma cells indirectly contributes to their proliferation.