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Anionic phospholipid‐induced regulation of reactive oxygen species production by human cytochrome P450 2E1
Author(s) -
Cho Eun Yi,
Yun Chul-Ho,
Chae Ho-Zoon,
Chae Han-Jung,
Ahn Taeho
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.04.048
Subject(s) - cardiolipin , cyp2e1 , phosphatidylcholine , reactive oxygen species , chemistry , phospholipid , biochemistry , enzyme , cytochrome p450 , oxygen , membrane , organic chemistry
We suggest that the cytochrome P450 2E1 (CYP2E1)‐induced formation of reactive oxygen species (ROS) can be regulated by anionic phospholipids and the presence of the N‐terminal region of the enzyme. When the content of cardiolipin (CL) in membranes at the expense of phosphatidylcholine matrix was increased, the ROS produced by recombinant human CYP2E1 was decreased as a function of CL concentration. On the contrary, the N‐terminally truncated CYP2E1 had a decreased effect on the lipid‐induced reduction of ROS formation. These results suggest that specific phospholipids can regulate the function of CYP2E1 by interaction with the enzyme including the N‐terminal region(s).