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Tissue‐specific expression of ALA synthase‐1 and heme oxygenase‐1 and their expression in livers of rats chronically exposed to ethanol
Author(s) -
Zheng Jianyu,
Tian Qing,
Hou Weihong,
Watts John A.,
Schrum Laura W.,
Bonkovsky Herbert L.
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.04.047
Subject(s) - heme oxygenase , heme , oxygenase , atp synthase , messenger rna , enzyme , biology , biochemistry , gene expression , ethanol , biosynthesis , microbiology and biotechnology , gene
5‐Aminolevulinic acid synthase‐1 (ALAS1) and heme oxygenase‐1 (HO‐1) are the rate‐controlling enzymes for heme biosynthesis and degradation, respectively. Expression of these two genes showed tissue‐specific expression pattern at both mRNA and protein levels in selected non‐treated rat tissues. In the livers of rats receiving oral ethanol for 10 weeks, ALAS1 mRNA levels were increased by 65%, and the precursor and mature ALAS1 protein levels were increased by 1.8‐ and 2.3‐fold, respectively, while no changes were observed in HO‐1 mRNA and protein levels, compared with pair‐fed controls. These results provide novel insights into the effects of chronic ethanol consumption on hepatic heme biosynthesis and porphyrias.