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Dissociation of superoxide production by mitochondrial complex I from NAD(P)H redox state
Author(s) -
Lambert Adrian J.,
Buckingham Julie A.,
Brand Martin D.
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.04.030
Subject(s) - superoxide , nad+ kinase , redox , chemistry , biochemistry , glycerol 3 phosphate dehydrogenase , mitochondrion , electron transport chain , photochemistry , enzyme , inorganic chemistry
The relationship between the rate of superoxide production by complex I and NAD(P)H redox state was investigated in rat skeletal muscle mitochondria. A high rate of superoxide production was observed during succinate oxidation; the rate during pyruvate oxidation was over fourfold lower. However, the NAD(P)H pool was significantly less reduced during succinate oxidation than during pyruvate oxidation. We conclude that there is no unique relationship between superoxide production by complex I and the reduction state of the NAD(P)H pool. Our data suggest that less than 10% of the superoxide originates from the flavin site during reverse electron transport from succinate.