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Down‐regulation of Notch‐dependent transcription by Akt in vitro
Author(s) -
Song Jieun,
Park Sungwoo,
Kim Minsoon,
Shin Incheol
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.04.024
Subject(s) - protein kinase b , microbiology and biotechnology , chemistry , phosphorylation , pi3k/akt/mtor pathway , repressor , transcription factor , biology , signal transduction , biochemistry , gene
The effect of Akt on Notch intracellular domain (NICD)‐mediated transcription was investigated. Transfection experiments revealed that constitutively active Akt down‐regulates NICD‐dependent transcription. Kinase inactive dominant negative Akt did not affect NICD transcriptional activity, indicating that Akt kinase activity is responsible for the down‐regulation. Studies using histone deacetylase (HDAC) and silencing mediator of retinoid and thyroid hormone receptor (SMRT) revealed that modulation of NICD transcriptional activity is not mediated by an HDAC‐dependent mechanism or recruitment of the co‐repressor SMRT. Akt inhibited proper nuclear localization of NICD, and phosphorylated NICD both in vitro and caused its hyperphosphorylation in vivo. These results may suggest possible regulation of NICD transcriptional activity by Akt‐mediated phosphorylation and subsequent inhibition of proper nuclear localization of NICD.