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A comparative metabolomic study of NHR‐49 in Caenorhabditis elegans and PPAR‐α in the mouse
Author(s) -
Atherton Helen J.,
Jones Oliver A.H.,
Malik Shahid,
Miska Eric A.,
Griffin Julian L.
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.04.020
Subject(s) - metabolomics , caenorhabditis elegans , metabolome , nuclear receptor , peroxisome , peroxisome proliferator activated receptor , biochemistry , fatty acid metabolism , peroxisome proliferator activated receptor alpha , glycolysis , gluconeogenesis , metabolic pathway , biology , fatty acid , alanine , receptor , lipid metabolism , chemistry , metabolism , amino acid , bioinformatics , gene , transcription factor
Proton Nuclear Magnetic Resonance spectroscopy and Gas Chromatography Mass Spectrometry based metabolomics has been used in conjunction with multivariate statistics to examine the metabolic changes in Caenorhabditis elegans following the deletion of nuclear hormone receptor‐49 ( nhr‐49 ). Deletion of the receptor produced profound changes in fatty acid metabolism, in particular an increase in the ratio of unsaturated to saturated fatty acids, a decrease in the concentration of glucose and increases in lactate and alanine. Given the proposed functional similarity between nhr‐49 and the mammalian peroxisome proliferator‐activated receptors (PPARs) these changes were compared with the metabolome of the PPAR‐α null mouse. The metabolomic approach demonstrated a number of similarities including the regulation of lipid synthesis, β‐oxidation of fatty acids and changes in glycolysis/gluconeogenesis.