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Proteins in the insulin‐secreting cell line MIN6 bind the imidazoline compound BL11282
Author(s) -
Shafqat Jawed,
Ishrat Moin,
Jägerbrink Theres,
Sillard Rannar,
Mäeorg Uno,
Efendic Suad,
Berggren Per-Olof,
Zaitsev Sergei V.,
Jörnvall Hans
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.04.007
Subject(s) - imidazoline receptor , band 3 , chemistry , islet , insulin , cell culture , biochemistry , secretion , membrane , membrane protein , biophysics , biology , endocrinology , genetics
The imidazoline BL11282 stimulates insulin release and alters islet proteomes. Subcellular fractions of MIN6 cells showed that the membrane fraction exhibited binding to BL11282 on a Biacore chip and to BL11282‐labelled magnetic beads. Bound material extracted from the beads showed a ∼50 kDa differential band upon SDS–PAGE and a weaker 100 kDa band. The former was sensitive to competitive removal by preincubation of the fraction with BL11282, then highlighting the ∼100 kDa band. Masspectrometric analysis revealed the ∼50 kDa band to be EF1A and the ∼100 kDa band to be glucose regulated P94, both of interest in insulin synthesis and secretion.