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One SmpB molecule accompanies tmRNA during its passage through the ribosomes
Author(s) -
Bugaeva Elizaveta Y.,
Shpanchenko Olga V.,
Felden Brice,
Isaksson Leif A.,
Dontsova Olga A.
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.03.049
Subject(s) - ribosome , translation (biology) , ef tu , messenger rna , ribosome profiling , chemistry , microbiology and biotechnology , rna , biochemistry , biology , biophysics , gene
tmRNA and SmpB are the main participants of trans ‐translation, a process which rescues the ribosome blocked during translation of non‐stop mRNA. While a one‐to‐one stoichiometry of tmRNA to the ribosome is generally accepted, the number of SmpB molecules in the complex is still under question. We have isolated tmRNA–ribosome complexes blocked at different steps of the tmRNA path through the ribosome and analyzed the stoichiometry of the complexes. Ribosome, tmRNA and SmpB were found in equimolar amount in the tmRNA–ribosome complexes stopped at the position of the 2nd, 4th, 5th or the 11th codons of the coding part of the tmRNA.